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primary antibody against serca2a  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc primary antibody against serca2a
    Primary Antibody Against Serca2a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+against+serca2a/pmc10202692__mmc2-519-6-49?v=Cell+Signaling+Technology+Inc
    Average 90 stars, based on 1 article reviews
    primary antibody against serca2a - by Bioz Stars, 2026-07
    90/100 stars

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    Protein expression of Ca2+ handling proteins. A: example Western blots of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA; 100 kDa), phospholamban (PLB; 6 kDa), the Ser16 phosphorylation site of PLB (SER16-PLB; 6 kDa), the Thr17 phosphorylation site of PLB (THR17-PLB; 6 kDa), the Na+-K+-ATPase (NA/K; 100 kDa), sodium-calcium exchanger (NCX; 120 kDa), ryanodine receptor (RYR; 565 kDa), and calsequestrin (CSQ; 50 kDa). Summary data of protein expression (relative to CSQ) of SERCA (B), PLB (C), SERCA/PLB ratio (D), SER16-PLB (E), THR17-PLB (F), Na+-K+ ATPase (G), NCX (H), and RYR (I). Data shown are from whole heart homogenates of CON, AB, and AB-LIT groups; n = 5–7 animals per group. *P < 0.05 vs. CON. #P < 0.05 vs. AB. §P < 0.10 vs. CON and AB.
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    Protein expression of Ca2+ handling proteins. A: example Western blots of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA; 100 kDa), phospholamban (PLB; 6 kDa), the Ser16 phosphorylation site of PLB (SER16-PLB; 6 kDa), the Thr17 phosphorylation site of PLB (THR17-PLB; 6 kDa), the Na+-K+-ATPase (NA/K; 100 kDa), sodium-calcium exchanger (NCX; 120 kDa), ryanodine receptor (RYR; 565 kDa), and calsequestrin (CSQ; 50 kDa). Summary data of protein expression (relative to CSQ) of SERCA (B), PLB (C), SERCA/PLB ratio (D), SER16-PLB (E), THR17-PLB (F), Na+-K+ ATPase (G), NCX (H), and RYR (I). Data shown are from whole heart homogenates of CON, AB, and AB-LIT groups; n = 5–7 animals per group. *P < 0.05 vs. CON. #P < 0.05 vs. AB. §P < 0.10 vs. CON and AB.
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    Protein expression of Ca2+ handling proteins. A: example Western blots of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA; 100 kDa), phospholamban (PLB; 6 kDa), the Ser16 phosphorylation site of PLB (SER16-PLB; 6 kDa), the Thr17 phosphorylation site of PLB (THR17-PLB; 6 kDa), the Na+-K+-ATPase (NA/K; 100 kDa), sodium-calcium exchanger (NCX; 120 kDa), ryanodine receptor (RYR; 565 kDa), and calsequestrin (CSQ; 50 kDa). Summary data of protein expression (relative to CSQ) of SERCA (B), PLB (C), SERCA/PLB ratio (D), SER16-PLB (E), THR17-PLB (F), Na+-K+ ATPase (G), NCX (H), and RYR (I). Data shown are from whole heart homogenates of CON, AB, and AB-LIT groups; n = 5–7 animals per group. *P < 0.05 vs. CON. #P < 0.05 vs. AB. §P < 0.10 vs. CON and AB.
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    Protein expression of Ca2+ handling proteins. A: example Western blots of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA; 100 kDa), phospholamban (PLB; 6 kDa), the Ser16 phosphorylation site of PLB (SER16-PLB; 6 kDa), the Thr17 phosphorylation site of PLB (THR17-PLB; 6 kDa), the Na+-K+-ATPase (NA/K; 100 kDa), sodium-calcium exchanger (NCX; 120 kDa), ryanodine receptor (RYR; 565 kDa), and calsequestrin (CSQ; 50 kDa). Summary data of protein expression (relative to CSQ) of SERCA (B), PLB (C), SERCA/PLB ratio (D), SER16-PLB (E), THR17-PLB (F), Na+-K+ ATPase (G), NCX (H), and RYR (I). Data shown are from whole heart homogenates of CON, AB, and AB-LIT groups; n = 5–7 animals per group. *P < 0.05 vs. CON. #P < 0.05 vs. AB. §P < 0.10 vs. CON and AB.

    Journal: Journal of Applied Physiology

    Article Title: Chronic low-intensity exercise attenuates cardiomyocyte contractile dysfunction and impaired adrenergic responsiveness in aortic-banded mini-swine

    doi: 10.1152/japplphysiol.00840.2017

    Figure Lengend Snippet: Protein expression of Ca2+ handling proteins. A: example Western blots of sarco/endoplasmic reticulum Ca2+ ATPase (SERCA; 100 kDa), phospholamban (PLB; 6 kDa), the Ser16 phosphorylation site of PLB (SER16-PLB; 6 kDa), the Thr17 phosphorylation site of PLB (THR17-PLB; 6 kDa), the Na+-K+-ATPase (NA/K; 100 kDa), sodium-calcium exchanger (NCX; 120 kDa), ryanodine receptor (RYR; 565 kDa), and calsequestrin (CSQ; 50 kDa). Summary data of protein expression (relative to CSQ) of SERCA (B), PLB (C), SERCA/PLB ratio (D), SER16-PLB (E), THR17-PLB (F), Na+-K+ ATPase (G), NCX (H), and RYR (I). Data shown are from whole heart homogenates of CON, AB, and AB-LIT groups; n = 5–7 animals per group. *P < 0.05 vs. CON. #P < 0.05 vs. AB. §P < 0.10 vs. CON and AB.

    Article Snippet: Blots were incubated overnight (25°C) with the primary antibody against SERCA2a (1:1,000; ThermoFisher Scientific), phospholamban (PLB; 1:1,000; ThermoFisher Scientific), sodium-calcium exchanger (NCX; 1:1,000; ThermoFisher Scientific), Na + -K + -ATPase (1:1,000; ThermoFisher Scientific), ryanodine receptor (RyR; 1:1,000; ThermoFisher Scientific), Ser16 phosphorylated PLB (1:1,000; Badrilla), Thr17 phosphorylated PLB (1:1,000; Badrilla), and calsequestrin (CSQ; 1:10,000; ThermoFisher Scientific).

    Techniques: Expressing, Western Blot, Phospho-proteomics